FastQCFastQC Report
Thu 8 Feb 2018
HWV77AFXX_n01_ah8867-55c-0218.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWV77AFXX_n01_ah8867-55c-0218.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9983276
Sequences flagged as poor quality0
Sequence length50
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC21700.018.14789644
GCACACG28450.017.16650611
ACACGTC30250.016.2182413
GATCGGA29950.016.0198331
GTCACAC30900.015.87700229
ACGTCTG31450.015.73932615
CGTCTGA32050.015.58196116
CACACGT31500.015.50481712
CACGTCT32600.015.04913614
CAGTCAC33050.015.04385227
AGATCGG23150.014.9261221
AGCACAC34150.014.36565210
CCAGTCA34900.014.30943626
TCCAGTC35700.014.23527425
AGTCACA36150.013.57121228
GAGCACA37050.013.5381049
TCGGAAG38450.013.2740993
AGAGCAC38350.012.9644558
ACTCCAG39950.012.61074623
CTCGTAT28650.012.59366642
Produced by FastQC (version 0.11.2)