FastQCFastQC Report
Thu 8 Feb 2018
HWV77AFXX_n01_ah8867-307i-0218.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWV77AFXX_n01_ah8867-307i-0218.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9003014
Sequences flagged as poor quality0
Sequence length50
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCTCGTA149250.16577781618466883TruSeq Adapter, Index 14 (97% over 44bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCTCGTAT106430.11821596634193837TruSeq Adapter, Index 14 (97% over 44bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGATCGG27800.026.835891
GCACACG36700.020.68101512
CTCGTAT27500.020.08048844
GATCGGA37650.019.7502652
ACACGTC38100.019.69011314
ACGTCTG38350.019.56175416
CGTCTGA38900.019.4548417
CACACGT38950.019.37338313
CACGTCT40350.018.70119515
GTTCCGT39950.018.44779236
GTCACAG40450.018.43720830
TCCAGTC40600.018.31510526
AGCACAC42500.018.16904611
TATCTCG38650.018.10134542
CCAGTCA41350.018.0893227
CCGTATC39900.018.02981439
CAGTCAC40750.017.97775328
AGTCACA42000.017.65222429
GAGCACA43300.017.52861410
CGTATCT41400.017.21781240
Produced by FastQC (version 0.11.2)