FastQCFastQC Report
Thu 8 Feb 2018
HWV77AFXX_n01_ah8867-307c-0218.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWV77AFXX_n01_ah8867-307c-0218.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6914275
Sequences flagged as poor quality0
Sequence length50
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCTTATCTCGTA86540.12516135097316783TruSeq Adapter, Index 20 (97% over 44bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCTTATCTCGTAT77810.1125352983501524TruSeq Adapter, Index 20 (97% over 44bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGATCGG20250.020.6479911
AGTCACG21750.018.61126329
CTCGTAT16850.018.41033444
CACGTGG22200.018.03594432
GCACACG22950.017.73398612
ACGTGGC22700.017.63867633
ACACGTC23350.017.5244114
GATCGGA24200.016.9090062
CACGTCT24900.016.61023715
CACACGT24350.016.53367613
ACGTCTG24950.016.48877316
GGCCTTA24100.016.15759537
TATCTCG24700.016.0331242
GTCACGT25700.015.92175330
CGTCTGA26550.015.66082517
TCCAGTC26550.015.57796426
GTGGCCT25800.015.51930135
TCACGTG26700.015.49022131
CAGTCAC26400.015.41647728
AGCACAC27000.015.31810911
Produced by FastQC (version 0.11.2)