FastQCFastQC Report
Thu 8 Feb 2018
HWV77AFXX_n01_ah8867-305i-0218.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWV77AFXX_n01_ah8867-305i-0218.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8746526
Sequences flagged as poor quality0
Sequence length50
%GC35

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG15500.021.57394412
ACACGTC16800.019.5116814
CACACGT17500.019.23406213
ACGTCTG17600.018.62478416
CACGTCT18600.017.62345115
GATCGGA18950.017.1821542
AGATCGG19450.016.9719161
CACAGTC19500.016.92268832
CGTCTGA19750.016.48588417
CAGTCAC20100.015.87046428
GAGCACA22250.015.72134810
CCAGTCA21250.015.21864827
AGCACAC22650.015.05484111
TCCAGTC21850.014.9014326
AGAGCAC22800.014.8596479
GTCACAG22300.014.60056130
AGTCACA23000.014.06070729
TCGGAAG24750.013.5999984
ACTCCAG25700.013.35393124
CTCCAGT26300.013.21657725
Produced by FastQC (version 0.11.2)