FastQCFastQC Report
Thu 8 Feb 2018
HWV77AFXX_n01_ah8867-305c-0218.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWV77AFXX_n01_ah8867-305c-0218.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4689622
Sequences flagged as poor quality0
Sequence length50
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATGC57860.1233788139001395TruSeq Adapter, Index 11 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCACGGC10650.026.44084530
CACGGCT10650.026.23427831
GTCACGG10750.025.78559129
CGGCTAC11450.024.78558733
AGTCACG11750.023.4038428
ACGGCTA12000.023.0995932
CATCTCG12300.022.17846939
CGTATGC12800.022.00078444
ACACGTC12900.021.14691413
GCACACG13350.020.7636811
CAGTCAC13900.020.57517427
GGCTACA13950.020.50142934
ACGTCTG13800.020.40543715
GATCGGA13500.020.051571
CACACGT14050.019.88576912
CACGTCT13750.019.8396514
CCAGTCA14300.019.53811626
CTCGTAT14200.019.52182242
ATCTCGT14050.019.10388440
TCGTATG14550.019.05222543
Produced by FastQC (version 0.11.2)