FastQCFastQC Report
Thu 8 Feb 2018
HWV77AFXX_n01_ah8867-303i-0218.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWV77AFXX_n01_ah8867-303i-0218.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5410897
Sequences flagged as poor quality0
Sequence length50
%GC35

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG8600.014.06940711
CGTATGC8850.013.92093344
ACACGTC9450.012.33853713
CACACGT10150.012.13784512
CGTCTGA10100.011.76229216
CACGTCT10550.011.26058314
GATCGGA10150.011.0571741
ACGTCTG10700.010.897119515
CAGTCAC11500.010.71296727
CCAGTCA11500.010.71296726
TCCAGTC12650.09.91297225
TCGTATG13150.09.70344343
AGTCACC12300.09.65846728
AGCACAC12400.09.58084210
GTCACCT12400.09.58057729
AGATCGG9300.09.4649221
GAGCACA13200.08.8335159
AGAGCAC13550.08.7677088
TCGGAAG13750.08.6397783
ACTCCAG14650.08.40949623
Produced by FastQC (version 0.11.2)