FastQCFastQC Report
Thu 8 Feb 2018
HWV77AFXX_n01_ah8867-303c-0218.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWV77AFXX_n01_ah8867-303c-0218.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7309538
Sequences flagged as poor quality0
Sequence length50
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGATCGG17700.022.1320321
GCACACG20400.018.44119312
CGTATGC13700.018.14637244
ACACGTC21150.017.99528514
GATCGGA21250.017.9104792
CACACGT21650.017.5796913
ACGTCTG21350.017.41453416
CACGTCT22300.016.57400515
CACTAGC23100.016.57110232
CGTCTGA23500.016.28937517
CAGTCAC24300.015.20978428
GAGCACA25950.014.9208110
TCACTAG26750.014.556622531
TCCAGTC25900.014.35512526
AGCACAC26850.014.25660411
ACTAGCT26700.014.254433
AGAGCAC27100.014.0440999
TATCTCG22950.013.99594140
CCAGTCA27550.013.97450627
CTAGCTT28350.013.73517934
Produced by FastQC (version 0.11.2)