FastQCFastQC Report
Thu 8 Feb 2018
HWV77AFXX_n01_ah8867-155i-0218.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWV77AFXX_n01_ah8867-155i-0218.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6724167
Sequences flagged as poor quality0
Sequence length50
%GC35

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATG79540.1182897450345894TruSeq Adapter, Index 7 (100% over 49bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGATCGG19750.023.8461421
GCACACG23250.020.43871712
CGTATGC15500.020.29753544
ACACGTC23650.019.90698414
GATCGGA25250.019.2550222
ACGTCTG24400.019.20492616
CACACGT24550.019.17719713
CGTCTGA25700.018.40467617
CACGTCT26000.018.19231415
AGCACAC26700.017.7972311
CAGTCAC26000.017.76910428
CATCTCG22100.017.6205740
AGTCACC27500.017.11987929
CCAGTCA27100.016.9666727
TCCAGTC28700.016.4807226
GAGCACA28950.016.4143910
AGAGCAC29350.016.2656429
CCAGATC28100.016.20592934
CTCGTAT24600.016.187643
TCGGAAG30700.015.6221544
Produced by FastQC (version 0.11.2)