FastQCFastQC Report
Thu 8 Feb 2018
HWV77AFXX_n01_ah8867-155c-0218.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWV77AFXX_n01_ah8867-155c-0218.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7741287
Sequences flagged as poor quality0
Sequence length50
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGC252660.32637983839121326TruSeq Adapter, Index 9 (100% over 50bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATG227320.29364626321178894TruSeq Adapter, Index 9 (100% over 49bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC37250.032.3661544
AGATCGG36600.031.1452671
CGATCAG59200.020.32731233
AGTCACG59450.020.27909728
GCACACG59950.020.10957511
ACACGTC60250.020.04647613
GTCACGA60000.020.01987329
ACGTCTG61300.019.66721315
CACGATC61750.019.66589231
CACGTCT61500.019.56748214
CGTCTGA62250.019.47309516
GATCTCG59600.019.37869639
GATCGGA61250.019.2216681
CACACGT63450.019.07013712
TCCAGTC64350.018.80329925
AGCACAC64900.018.71174610
CAGTCAC65350.018.61656227
GAGCACA65600.018.5791519
TCACGAT66100.018.3384130
ATCTCGT63450.018.27324940
Produced by FastQC (version 0.11.2)