FastQCFastQC Report
Thu 8 Feb 2018
HWV77AFXX_n01_ah7797-f30i-0218.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWV77AFXX_n01_ah7797-f30i-0218.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8499237
Sequences flagged as poor quality0
Sequence length50
%GC35

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATG182680.21493694081009862TruSeq Adapter, Index 2 (100% over 49bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGATCGG30000.029.1970231
GCACACG32950.026.44010712
GATCGGA34800.025.2238852
ACACGTC34800.025.09774414
ACGTCTG34950.024.9900316
CACACGT35150.024.91042913
CGTCTGA35700.024.58827617
CACGTCT36200.023.88401815
GTCACCG36600.023.80261830
CCAGTCA37200.023.71495627
CCGATGT36750.023.70546734
CAGTCAC37150.023.68765628
CGATGTA37100.023.54112635
TCCAGTC37150.023.3915626
TATCTCG34650.023.1118340
AGTCACC38900.022.50890529
ACCGATG39150.022.4770333
CACCGAT39300.022.39123732
AGCACAC39100.022.16818211
TCGTATG37650.021.32868644
Produced by FastQC (version 0.11.2)