FastQCFastQC Report
Thu 8 Feb 2018
HWV77AFXX_n01_ah7797-f30c-0218.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWV77AFXX_n01_ah7797-f30c-0218.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6150901
Sequences flagged as poor quality0
Sequence length50
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCCGATCTCGTA244010.3967061085847423TruSeq Adapter, Index 16 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCCGATCTCGTAT76560.12446956958013142TruSeq Adapter, Index 16 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGATCGG36800.034.3866771
CGTCCCG40800.029.71024736
GTCCCGA39900.029.00198237
ACCCGTC42450.028.55543333
CCCGTCC42900.028.20461834
GCACACG44750.028.12084812
TCACCCG43500.028.06822831
CACCCGT43800.027.8762132
ACGTCTG45900.027.46422616
CCGTCCC44300.027.26361535
ACACGTC46300.027.22695414
CCCGATC38550.027.22107739
GATCGGA46850.027.1418912
CACGTCT46700.027.08796315
CGTCTGA46900.026.9255417
GTCACCC45650.026.84266930
CACACGT47350.026.62318613
AGCACAC47600.026.62115111
CCGATCT39900.026.3562740
CGATCTC40550.026.20506341
Produced by FastQC (version 0.11.2)