FastQCFastQC Report
Thu 8 Feb 2018
HWV77AFXX_n01_ah7797-5i-0218.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWV77AFXX_n01_ah7797-5i-0218.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7282034
Sequences flagged as poor quality0
Sequence length50
%GC35

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATG110690.15200423398187923TruSeq Adapter, Index 4 (100% over 49bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG18650.027.01301812
ACACGTC19650.025.63830814
ACTGACC20450.024.52776333
CACACGT20650.024.29020913
AGATCGG21200.023.9790921
GATCGGA21400.023.7478122
CACGTCT21750.023.36518115
ACGTCTG21250.023.19025616
CAGTCAC21950.022.75153728
CGTCTGA22600.021.9023417
CTGACCA23400.021.24755934
AGCACAC24050.020.9475811
TCGTATG24100.020.72311644
GAGCACA24800.020.66891710
TCCAGTC24300.020.64181926
CTCGTAT23650.020.55925643
CCAGTCA24500.020.29372427
AGAGCAC26350.019.4530969
CACTGAC25450.019.44962332
ATCTCGT24600.019.31812341
Produced by FastQC (version 0.11.2)