FastQCFastQC Report
Thu 8 Feb 2018
HWV77AFXX_n01_ah7797-5c-0218.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWV77AFXX_n01_ah7797-5c-0218.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5976064
Sequences flagged as poor quality0
Sequence length50
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCCG5850.015.41859533
CGTCCGC6850.012.84654134
CACGTCC8650.011.190632
CCGCACA7750.011.07081837
AGTCACG8350.010.8023429
GTCCGCA8800.010.74985435
ACACGTC9100.010.63730814
GCACACG9500.010.42099912
CACACGT10200.010.35288813
AGATCGG9600.010.0865731
GTCACGT9300.09.93526630
TCCGCAC9150.09.61735636
GATCGGA11600.09.1034022
TCACGTC10150.09.1032531
CGCACAT11350.08.72234938
CATCTCG10100.08.71319242
ACGTCTG10700.08.63546916
CACGTCT10950.08.43831315
CAGTCAC11750.08.42548928
AGCACAC13250.08.13567111
Produced by FastQC (version 0.11.2)