FastQCFastQC Report
Thu 8 Feb 2018
HWV77AFXX_n01_ah7797-30i-0218.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWV77AFXX_n01_ah7797-30i-0218.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6966735
Sequences flagged as poor quality0
Sequence length50
%GC35

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATG151280.21714619545597758TruSeq Adapter, Index 5 (100% over 49bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGC109720.15749127819559666TruSeq Adapter, Index 5 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGATCGG30850.031.5310611
CGTATGC24800.028.38765144
GCACACG43650.021.92435612
ACACGTC44150.021.82555214
CACGTCT45000.021.4621815
ACGTCTG45100.021.41459316
GATCGGA45850.021.304212
CACACGT45350.021.15100513
CACACAG45650.020.96351432
CGTCTGA46300.020.8595717
GTCACAC46200.020.85680430
CACAGTG46950.020.38305534
CAGTCAC47500.020.23981528
GATCTCG35450.020.04552740
TCCAGTC48150.020.0122826
AGCACAC48150.019.96644411
CCAGTCA48050.019.91657327
GAGCACA49400.019.77295710
ACACAGT48750.019.76583133
AGAGCAC49050.019.689799
Produced by FastQC (version 0.11.2)