FastQCFastQC Report
Thu 8 Feb 2018
HWV77AFXX_n01_ah7797-30c-0218.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWV77AFXX_n01_ah7797-30c-0218.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8502737
Sequences flagged as poor quality0
Sequence length50
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAACGATCTCGTA632250.7435840953330675TruSeq Adapter, Index 19 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAACGATCTCGTAT467320.5496112604682469TruSeq Adapter, Index 19 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGATCGG83850.037.95221
CTCGTAT68900.035.2202744
AGTCACG134250.023.4664429
GATCGGA136950.023.245032
GCACACG137150.023.1790512
ACACGTC137200.023.0904314
GTCACGT136500.023.06310830
CACACGT138300.022.9385913
ACGTCTG138350.022.930316
CACGTCT139250.022.82949315
TCACGTG138000.022.76459731
CGTCTGA139500.022.75703817
CACGTGA138650.022.7216132
CGATCTC126450.022.65314941
AGCACAC141100.022.60746211
CAGTCAC139900.022.55017728
GATCTCG128350.022.523542
CCAGTCA140450.022.43054227
TCCAGTC141050.022.28833826
GAGCACA143100.022.26101110
Produced by FastQC (version 0.11.2)