FastQCFastQC Report
Thu 5 Apr 2018
HWV57AFXX_n01_ah9847i3h-0318.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWV57AFXX_n01_ah9847i3h-0318.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6903939
Sequences flagged as poor quality0
Sequence length100
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATGC99080.14351227610788567TruSeq Adapter, Index 11 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCACGGC35900.023.17190230-31
AGGGGGG25750.021.44671672-73
ACGGCTA39150.021.36835732-33
TGCCGTC38350.021.26370648-49
AAGGGGG29850.021.0989472-73
AGTCACG40250.020.95944828-29
GAGCACA70400.019.8272979
CACGGCT34450.019.71339830-31
CGTATGC42500.019.51892744-45
TATGCCG43550.019.15638246-47
GTCACGG35650.018.98384928-29
GCCGTCT35550.018.64165148-49
CGGCTAC36400.018.59276432-33
GGCTACA42100.018.53142734-35
AGAGCAC76950.018.1395938
CATCTCG31550.018.02473438-39
CTCGTAT43250.017.92995342-43
GTATGCC39900.017.19808644-45
ATCTCGT41250.017.0333540-41
CCAGTCA51250.017.01124426-27
Produced by FastQC (version 0.11.2)