FastQCFastQC Report
Thu 5 Apr 2018
HWV57AFXX_n01_ah9847c3hmyc-0318.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWV57AFXX_n01_ah9847c3hmyc-0318.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6115367
Sequences flagged as poor quality0
Sequence length100
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGC122040.19956283899232866TruSeq Adapter, Index 6 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCACA54700.027.1505839
TCACGCC31800.026.97212630-31
AGTCACG33000.026.27594228-29
AGAGCAC58250.025.5765978
GTCACGC28750.025.25611328-29
CGTATGC35450.023.93143844-45
TGCCGTC34300.023.84223648-49
CACGCCA32100.022.6937430-31
AAGGGGG31200.022.37207872-73
ATCTCGT38300.021.59761240-41
TATGCCG39000.021.51212146-47
CGTCTGA41950.021.22997916-17
GCCGTCT32800.021.20702448-49
CTCGTAT38950.021.1772942-43
GATCGGA65350.021.0759281
CACGTCT42850.020.67439814-15
GTATGCC34150.020.57587644-45
TCGGAAG72050.020.4833013
AGGGGGG30350.020.28836672-73
CCAGTCA43050.020.25092526-27
Produced by FastQC (version 0.11.2)