FastQCFastQC Report
Thu 5 Apr 2018
HWV57AFXX_n01_ah9767i3h-0318.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWV57AFXX_n01_ah9767i3h-0318.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6709235
Sequences flagged as poor quality0
Sequence length100
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGC82830.12345669811833987TruSeq Adapter, Index 9 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC34800.018.77323548-49
GCCGTCT31950.017.5792648-49
AGGGGGG25650.017.40750572-73
CGTATGC38500.017.09119644-45
AGTCACG38800.016.958128-29
TATGCCG39500.016.59901446-47
AAGGGGG31300.016.51766472-73
CTCGTAT38500.015.74790642-43
CGATCAG34900.015.62122332-33
CACGATC37150.015.37098130-31
CCGTCTT44200.014.99324250-51
GATCTCG30950.014.50196538-39
GTATGCC38250.014.25383544-45
GTCACGA38850.013.97242728-29
TCACGAT47450.013.96583930-31
ATGCCGT39050.013.96182346-47
ATCTCGT40350.013.0456340-41
CCAGTCA53350.012.59744626-27
TCGTATG44600.012.59293342-43
GAGCACA91450.012.2830099
Produced by FastQC (version 0.11.2)