FastQCFastQC Report
Thu 5 Apr 2018
HWV57AFXX_n01_ah9767c3htop2-spikein-0318.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWV57AFXX_n01_ah9767c3htop2-spikein-0318.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5981760
Sequences flagged as poor quality0
Sequence length100
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATG83420.13945728347509762TruSeq Adapter, Index 4 (100% over 49bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTGACC32300.023.13531132-33
TGCCGTC33600.021.96099348-49
CGTATGC36500.020.66751744-45
AGAGCAC63650.019.7145259
CGTCTGA42250.018.91018116-17
TATGCCG40900.018.67402646-47
CCAGTCA42750.018.52433826-27
GCCGTCT31850.018.37179250-51
GATCGGA67800.018.0242422
AGTCACT44950.017.82688328-29
CTCGTAT37900.017.29951342-43
CACGTCT46450.017.14973414-15
GTATGCC35000.017.12182646-47
CCGTCTT44800.017.04774750-51
AGATCGG72550.016.9761411
AAGGGGG34100.016.88519172-73
TCGGAAG74650.016.4960984
ATCTCGT36050.016.36169240-41
CACACGT49450.016.34690712-13
CTCCAGT50100.015.90043824-25
Produced by FastQC (version 0.11.2)