FastQCFastQC Report
Thu 5 Apr 2018
HWV57AFXX_n01_ah9624c3hflag-0318.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWV57AFXX_n01_ah9624c3hflag-0318.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6613915
Sequences flagged as poor quality0
Sequence length100
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC28850.017.26954848-49
CGTATGC31550.015.94020444-45
TATGCCG32150.015.64289646-47
AAGGGGG26500.014.45482972-73
AGTCACC38150.013.36636928-29
CCGTCTT38500.012.8797150-51
CTCGTAT33950.012.80511542-43
CCAGTCA41500.012.23077326-27
ATCTCGT34750.012.0368240-41
CGTCTGA45150.011.34585316-17
AGGGGGG21850.011.29299372-73
CACGTCT48750.010.74897414-15
GCCGTCT26100.010.71512848-49
CTCCAGT50950.010.42343424-25
CACACGT53050.09.8778212-13
ACCTTGT54350.09.51213432-33
GTATGCC31000.09.47605944-45
TCTGAAC58450.09.16625718-19
TGAACTC55100.09.08384420-21
TGCTTGA56800.08.85437758-59
Produced by FastQC (version 0.11.2)