FastQCFastQC Report
Thu 5 Apr 2018
HWV57AFXX_n01_ah9621i3h-0318.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWV57AFXX_n01_ah9621i3h-0318.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7308027
Sequences flagged as poor quality0
Sequence length100
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCTTATCTCGTA76000.10399523701814456TruSeq Adapter, Index 20 (97% over 44bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC36100.019.72451650-51
ACGTGGC37000.018.86261432-33
TATGCCG39700.018.17279848-49
AGTCACG40900.017.58094828-29
CGTATGC40650.017.5166146-47
GGCCTTA40700.016.74401936-37
CTCGTAT40750.016.4933144-45
AAGGGGG30600.016.35829474-75
GTGGCCT47050.015.08323434-35
TCACGTG47700.015.07470230-31
CCGTCTT46200.014.90373352-53
GCCGTCT34250.014.54603952-53
ATCTCGT44400.014.34307942-43
GTATGCC38750.013.28147548-49
AGGGGGG27100.013.26784976-77
CCAGTCA55650.013.21675526-27
CACGTGG37650.012.98211632-33
TATCTCG37200.012.12870342-43
ATGCCGT44150.011.60370550-51
CGTGGCC41400.011.57915934-35
Produced by FastQC (version 0.11.2)