FastQCFastQC Report
Thu 5 Apr 2018
HWV57AFXX_n01_ah9621c3hflag-0318.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWV57AFXX_n01_ah9621c3hflag-0318.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7602897
Sequences flagged as poor quality0
Sequence length100
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC32200.019.3400348-49
CGTATGC35650.017.79841844-45
AAGGGGG31350.016.41651272-73
TATGCCG39750.016.19887546-47
CCAGTCA46350.015.61558226-27
CCGTCTT42400.014.29950650-51
AGTCACC48850.014.23915828-29
CTCGTAT41150.013.59176142-43
AGGGGGG26550.013.188519572-73
CGTCTGA55900.012.77931316-17
ATCTCGT40950.012.73990640-41
CATCTCG28900.012.27844538-39
GTATGCC34650.011.80111944-45
GCCGTCT32100.011.78658648-49
TCACCAG59000.011.74986630-31
CTCCAGT60500.011.73025124-25
CACGTCT61500.011.5392514-15
CAGATCA59550.011.32564734-35
CACACGT67000.010.7323612-13
TCTGAAC69150.010.60249718-19
Produced by FastQC (version 0.11.2)