FastQCFastQC Report
Thu 5 Apr 2018
HWV57AFXX_n01_ah9012i3h-0318.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWV57AFXX_n01_ah9012i3h-0318.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7068550
Sequences flagged as poor quality0
Sequence length100
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGTGGATATCTCGTAT187240.2648916680224374TruSeq Adapter, Index 7 (97% over 36bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGTGGATATCTCGTA104020.14715889397401163TruSeq Adapter, Index 7 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGAGTG57900.029.79044532-33
TGCCGTC57250.029.431650-51
CGTATGC61750.028.16219746-47
AGTCACG61500.027.96959528-29
AAGGGGG47100.027.2420974-75
TCACGAG63650.027.09924130-31
TATGCCG63300.026.98987648-49
CTCGTAT62250.026.69019944-45
CCGTCTT67400.024.79028152-53
GAGTGGA70850.024.5111734-35
ATCTCGT67100.024.06014642-43
CCAGTCA77400.022.37602426-27
GTGGATA77600.021.9552736-37
AGGGGGG43800.021.13930974-75
GAGCACA111350.020.4712359
CGTCTGA85800.020.40389616-17
GGATATC79550.020.20548438-39
GCCGTCT55550.019.925450-51
CGAGTGG56500.019.92262332-33
CACGAGT56650.019.74542630-31
Produced by FastQC (version 0.11.2)