FastQCFastQC Report
Thu 5 Apr 2018
HWV57AFXX_n01_ah9012c3hflag-0318.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWV57AFXX_n01_ah9012c3hflag-0318.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7140521
Sequences flagged as poor quality0
Sequence length100
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCTCGTA222250.3112517980130582TruSeq Adapter, Index 14 (97% over 44bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCTCGTAT198030.2773327044343123TruSeq Adapter, Index 14 (97% over 44bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC70000.035.4855450-51
TATGCCG75650.033.17720448-49
CCGTATC71200.032.5092138-39
AGTTCCG81100.032.3655134-35
CGTATGC77850.032.23907546-47
AAGGGGG63000.031.2222374-75
CTCGTAT77300.030.09706144-45
CCAGTCA88700.030.06944326-27
AGTCACA89350.030.00849728-29
CGTCTGA90200.029.95976416-17
ATCTCGT75550.029.61169442-43
CCGTCTT83000.029.13477752-53
CACGTCT94050.028.83328414-15
CACACGT94300.028.70740712-13
TTCCGTA90950.028.16257936-37
TCACAGT96350.027.73096530-31
CTCCAGT99200.027.07620224-25
TGAACTC101800.026.5924220-21
ACAGTTC100550.026.5491732-33
GTATCTC87250.026.01754840-41
Produced by FastQC (version 0.11.2)