FastQCFastQC Report
Thu 5 Apr 2018
HWV57AFXX_n01_ah8867b-0318.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWV57AFXX_n01_ah8867b-0318.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6416358
Sequences flagged as poor quality0
Sequence length100
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAACGATCTCGTAT194010.3023677918220897TruSeq Adapter, Index 19 (97% over 40bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAACGATCTCGTA121270.18900129949108202TruSeq Adapter, Index 19 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGGGGG55250.033.21956374-75
TGCCGTC69600.032.98821350-51
AGTCACG73250.032.43371628-29
TATGCCG71350.032.34404848-49
CGATCTC56650.032.14857540-41
CGTATGC72900.031.91461446-47
CCGTCTT76900.029.7650152-53
CTCGTAT71650.029.68321644-45
TCACGTG82150.028.80502730-31
ATCTCGT64600.028.5559842-43
CCAGTCA84700.028.16009726-27
CGTCTGA89100.026.92677316-17
ACGTGAA89400.026.81075532-33
CACGTCT90300.026.43883114-15
AACGATC71150.026.25722338-39
CTCCAGT91750.026.12415124-25
GAGCACA108250.025.745419
CACACGT94850.025.12138712-13
GTGAAAC94200.025.09536234-35
AGAGCAC114250.024.516768
Produced by FastQC (version 0.11.2)