FastQCFastQC Report
Thu 5 Apr 2018
HWV57AFXX_n01_ah8867a-0318.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWV57AFXX_n01_ah8867a-0318.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6997058
Sequences flagged as poor quality0
Sequence length100
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCGCACATCTCGTAT90110.12878269695634936TruSeq Adapter, Index 18 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCCG42200.023.3327232-33
TGCCGTC43100.022.57295450-51
GTCCGCA44750.021.79316934-35
CCGCACA42950.021.55734836-37
TATGCCG45650.021.41483948-49
AAGGGGG33950.020.6966474-75
CGTATGC46450.020.43877246-47
CCGTCTT49750.019.31955552-53
AGTCACG52000.019.16101628-29
CTCGTAT46200.018.66737444-45
TCACGTC55600.018.0896330-31
GCACATC47250.017.20793538-39
ATCTCGT48850.016.21131542-43
CCAGTCA67250.015.02572126-27
ACATCTC54100.014.98562140-41
AGGGGGG30050.014.232964574-75
CGTCTGA74950.013.54437216-17
GCCGTCT39600.013.53022450-51
TGCTTGA68700.013.3408260-61
CTCCAGT77800.013.19960624-25
Produced by FastQC (version 0.11.2)