FastQCFastQC Report
Thu 5 Apr 2018
HWV57AFXX_n01_ah7797i3h-0318.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWV57AFXX_n01_ah7797i3h-0318.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5726906
Sequences flagged as poor quality0
Sequence length100
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGGGGG18050.012.49911172-73
TGCCGTC22150.012.41293548-49
ACTTAGG24750.011.39364532-33
CGTATGC27000.010.96676544-45
CTCGTAT26300.010.18627142-43
TATGCCG28350.010.11301146-47
TTAGGCA29250.010.04243234-35
AGGGGGG15750.09.69879772-73
GCCGTCT19500.09.52039148-49
CCGTCTT30150.08.80759350-51
CTTAGGC22300.08.7464132-33
ATCTCGT28350.08.62070240-41
GCATCTC27350.08.59213838-39
CATCTCG18750.08.52248438-39
AGGCATC28850.08.22689536-37
GTATGCC24200.08.0599944-45
AGTCACT37650.07.989134328-29
ATGCCGT25950.07.78815946-47
CCAGTCA38450.07.639494426-27
TAGGCAT27150.06.924284534-35