FastQCFastQC Report
Thu 5 Apr 2018
HWV57AFXX_n01_ah7797c3htop2-spikein-0318.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWV57AFXX_n01_ah7797c3htop2-spikein-0318.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6973530
Sequences flagged as poor quality0
Sequence length100
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC28300.011.70848250-51
CGTATGC30800.011.06359146-47
CGTCTGA34500.010.14880416-17
CCAGTCA36500.09.65729326-27
CACGTCT37000.09.5901314-15
ATCTCGT33100.09.58449142-43
CTCGTAT36350.09.50364444-45
TATGCCG36400.09.49035348-49
AGTCACA40100.08.90747628-29
CACACGT39300.08.72993812-13
AAGGGGG31600.08.40351274-75
CCGTCTT38350.08.39501752-53
ACATTCC47250.08.00720832-33
TGAACTC48750.07.9053320-21
GAGCACA53600.07.6284329
CTCCAGT48150.07.41827224-25
AGGGGGG23200.07.29313174-75
GCCGTCT26100.07.293104650-51
AGCACAC50000.07.237715210-11
TCTGAAC52600.07.103282518-19
Produced by FastQC (version 0.11.2)