FastQCFastQC Report
Thu 5 Apr 2018
HWV57AFXX_n01_ah7797c0htop2-spikein-0318.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWV57AFXX_n01_ah7797c0htop2-spikein-0318.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7955063
Sequences flagged as poor quality0
Sequence length100
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC30500.015.33278750-51
CGTATGC33900.014.07240446-47
AGGGGGG26650.012.34575874-75
CTCGTAT40350.011.76474544-45
AAGGGGG32100.011.64069974-75
GTCACAC32550.011.62314428-29
TATGCCG40500.011.60493948-49
GCCGTCT29800.011.59228250-51
AGTCACA43750.011.49435128-29
GTATGCC32750.011.33751146-47
ATCTCGT39950.011.23522442-43
CCAGTCA42950.011.10671726-27
GAGCACA68000.010.7126289
ACACTGA46500.010.4608332-33
ATGCCGT36400.010.39423148-49
TATCTCG35100.010.37723340-41
CGTCTGA46950.010.16010716-17
TCACACT48450.010.0883730-31
AGAGCAC74550.010.0866188
CACGTCT47850.010.01811614-15
Produced by FastQC (version 0.11.2)