FastQCFastQC Report
Thu 5 Apr 2018
HWV57AFXX_n01_ah6408i-0318.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWV57AFXX_n01_ah6408i-0318.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5899982
Sequences flagged as poor quality0
Sequence length100
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCCGATCTCGTA200060.3390857802617025TruSeq Adapter, Index 16 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCCGATCTCGTAT90990.15422080948721537TruSeq Adapter, Index 16 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCCGA57850.038.34615336-37
AGATCGG105950.037.8003121
AGAGCAC109250.036.7380379
ACCCGTC71500.035.23237632-33
TCACCCG72100.035.00421530-31
TGCCGTC71500.034.543850-51
TCGGAAG122000.033.0931854
GATCGGA121250.033.0664252
CCCGATC50500.033.03876538-39
CGATCTC49350.032.8089740-41
CGGAAGA124750.032.4393045
CGTATGC79050.032.344546-47
CCGTCCC79450.031.88438834-35
ATCGGAA130250.030.9249043
TATGCCG82850.030.54884348-49
AGTCACC83100.030.39896228-29
CTCGTAT69800.030.36845444-45
CCAGTCA85150.029.91585726-27
AAGGGGG56850.029.88806774-75
CACGTCT90050.029.8008214-15
Produced by FastQC (version 0.11.2)