FastQCFastQC Report
Thu 5 Apr 2018
HWV57AFXX_n01_ah6408a-0318.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWV57AFXX_n01_ah6408a-0318.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8071917
Sequences flagged as poor quality0
Sequence length100
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCTCGTA194990.2415659130290859TruSeq Adapter, Index 15 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCTCGTAT116350.14414171999043102TruSeq Adapter, Index 15 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC65100.029.42076750-51
AAGGGGG56950.028.39078974-75
TATGCCG71250.027.87095648-49
CGTATGC73250.026.91772846-47
CTCGTAT66750.026.37019244-45
ATCTCGT75550.023.35974342-43
CCGTCTT76850.023.11812452-53
TCACATG102800.022.17337430-31
ATGTCAG103800.022.09545334-35
ACATGTC105050.021.74326332-33
AGGGGGG51800.021.73130676-77
AGTCACA107450.021.23534228-29
CCAGTCA109500.020.73054926-27
GAATCTC91400.019.46256840-41
GTCAGAA114100.019.11229336-37
GCCGTCT59400.018.99019852-53
GTATGCC69250.018.5290448-49
CTCCAGT126400.018.0145424-25
ATGCCGT72750.017.50828750-51
TGCTTGA94100.017.43286360-61
Produced by FastQC (version 0.11.2)