FastQCFastQC Report
Thu 5 Apr 2018
HWV57AFXX_n01_ah5184c3htop2-spikein-0318.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWV57AFXX_n01_ah5184c3htop2-spikein-0318.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6703398
Sequences flagged as poor quality0
Sequence length100
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATG88200.13157506088703072TruSeq Adapter, Index 2 (100% over 49bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGC78030.11640365080515883TruSeq Adapter, Index 2 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC41200.024.29891648-49
CGATGTA48900.022.15382834-35
ACCGATG50400.022.10045832-33
CGTATGC48250.021.72212644-45
CCAGTCA52200.021.47382726-27
CGTCTGA52350.021.27691316-17
AGTCACC53800.020.70377328-29
TCACCGA54400.020.60493930-31
CTCGTAT48900.020.27964242-43
CACGTCT55550.020.05116714-15
TATGCCG52400.020.0469946-47
ATCTCGT46250.019.8155640-41
AAGGGGG43500.019.2326272-73
CCGTCTT51800.018.82750950-51
CACACGT58950.018.73524712-13
CTCCAGT60450.018.5042824-25
TGAACTC62900.017.78319520-21
TCTGAAC65350.017.36801318-19
AGCACAC66050.017.2199110-11
AGAGCAC73600.016.8583249
Produced by FastQC (version 0.11.2)