FastQCFastQC Report
Thu 5 Apr 2018
HWV57AFXX_n01_ah3988c3hflag-0318.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWV57AFXX_n01_ah3988c3hflag-0318.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8160185
Sequences flagged as poor quality0
Sequence length100
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC36050.017.99170150-51
TATGCCG39700.016.51518648-49
CGTATGC40000.016.45017446-47
CTCGTAT39750.015.2529944-45
AAGGGGG34100.014.886106574-75
CCGTCTT48050.013.59631352-53
ATCTCGT45000.013.05553242-43
CCAGTCA53550.012.90154426-27
AGTCACA54750.012.4042428-29
AGTCAAC57950.012.20611634-35
CGTCTGA55850.012.117558516-17
TCACAGT58450.011.73992730-31
CACGTCT60050.011.38743214-15
ACAGTCA61900.011.23739132-33
GCCGTCT33700.010.94811952-53
AGGGGGG26900.010.92012276-77
CTCCAGT63650.010.89117424-25
CACACGT64350.010.772634512-13
TGAACTC72750.09.85176420-21
TCTGAAC73450.09.72579118-19
Produced by FastQC (version 0.11.2)