FastQCFastQC Report
Thu 5 Apr 2018
HWV57AFXX_n01_ah119i3h-0318.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWV57AFXX_n01_ah119i3h-0318.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6461987
Sequences flagged as poor quality0
Sequence length100
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC23450.09.21965748-49
AAGGGGG20650.08.30786472-73
TATGCCG26250.08.14695446-47
CGTATGC25800.07.74252244-45
AGTCACT36550.06.686397628-29
CTCGTAT28200.06.666773342-43
GCCGTCT21100.06.571118448-49
CCAGTCA35600.06.46877926-27
CCGTCTT33800.06.32695150-51
ACTAGCT34300.06.302844532-33
TCACTAG36600.06.22783230-31
ATCTCGT30350.06.19437840-41
TAGCTTA40600.06.077236734-35
TAGATCG38100.06.04459994
AGGGGGG18100.05.97263372-73
CGTCTGA41300.05.57590716-17
CTCCAGT43450.05.57051524-25
AGATCGG88000.05.55452794
GCTTATC37500.05.45187636-37
TATCTCG23300.05.345392738-39