FastQCFastQC Report
Thu 5 Apr 2018
HWV57AFXX_n01_ah119c3htop2-0318.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWV57AFXX_n01_ah119c3htop2-0318.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6431070
Sequences flagged as poor quality0
Sequence length100
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATG121680.1892064617551978TruSeq Adapter, Index 5 (100% over 49bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGC111520.1734081575849742TruSeq Adapter, Index 5 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC59450.031.3468348-49
CGTATGC64350.028.99618744-45
CCAGTCA71450.028.21881726-27
TATGCCG67700.027.87399546-47
ACACAGT74150.026.93807832-33
AGTCACA74250.026.9333428-29
AAGGGGG57800.026.7938472-73
CGTCTGA75650.026.68247816-17
CCGTCTT69800.025.89066750-51
CTCGTAT59200.025.88101842-43
CACGTCT79500.025.59711314-15
CTCCAGT81100.025.00567824-25
ATCTCGT54200.024.58305240-41
AGTGATC57400.024.31821436-37
CACACGT85350.023.78768512-13
TCACACA84250.023.54144330-31
TCTGAAC86950.023.45814918-19
TGAACTC86400.023.2812920-21
TGCTTGA77200.022.52657758-59
TGATCTC61450.022.17991338-39
Produced by FastQC (version 0.11.2)