FastQCFastQC Report
Thu 21 Nov 2019
HWV52AFXY_n02_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWV52AFXY_n02_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26909463
Sequences flagged as poor quality0
Sequence length90
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGATCCGACTCGGTGCCACTTTTTCAACTAGTTCCGCTTACACAGCTGAC12885064.788300680693628No Hit
ATCGATCCGACTCGGTGCCACTTTTTCAACTAGTTCCGCTTACACAGCTG5448592.0247858532145364No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3879021.441507769961816No Hit
TCGATCCGACTCGGTGCCACTTTTTCAACTAGTTCCGCTTACACAGCTGA3578291.3297515450233994No Hit
GATCCGACTCGGTGCCACTTTTTCAACTAGTTCCGCTTACACAGCTGACG2109670.7839881457314849No Hit
ATCGATCCGACTCGGTGCCACTTTTTCAACTAGTTCCGCTTACACAGCTT2000660.7434782329175428No Hit
ATCGATCCGACTCGGTGCCACTTTTTCAACTAGTTCCGCTTACACAGCTA1938210.7202707835529829No Hit
ATCCGACTCGGTGCCACTTTTTCAACTAGTTCCGCTTACACAGCTGACGC1893400.7036186489488846No Hit
ATCGATCCGACTCGGTGCCACTTTTTCAACTAGTTCCGCTTACACAGCTC1575080.5853256900741571No Hit
TCCGACTCGGTGCCACTTTTTCAACTAGTTCCGCTTACACAGCTGACGCT1464230.5441320029314595No Hit
TCGATCCGACTCGGTGCCACTTTTTCAACTAGTTCCGCTTACACAGCTTG685080.2545870201869134No Hit
TCGATCCGACTCGGTGCCACTTTTTCAACTAGTTCCGCTTACACAGCTAT678630.2521900938714384No Hit
TCGATCCGACTCGGTGCCACTTTTTCAACTAGTTCCGCTTACACAGCTAA664690.24700976009814837No Hit
TCGATCCGACTCGGTGCCACTTTTTCAACTAGTTCCGCTTACACAGCTTA628060.23339744832514864No Hit
TCGATCCGACTCGGTGCCACTTTTTCAACTAGTTCCGCTTACACAGCTTT621430.23093363104273024No Hit
GGGGGGGGGGGGGGGGGGGGTTGGGGGGGGGGGGGGGGGGGGGGGGGGGG619830.2303390446698992No Hit
TCGATCCGACTCGGTGCCACTTTTTCAACTAGTTCCGCTTACACAGCTAG616890.22924649220982227No Hit
GGGGGGGGGGGGGGGGGGGGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGG611590.22727692484981957No Hit
CGATCCGACTCGGTGCCACTTTTTCAACTAGTTCCGCTTACACAGCTTGT608220.22602457730204426No Hit
TCGATCCGACTCGGTGCCACTTTTTCAACTAGTTCCGCTTACACAGCTAC570120.2118659892990061No Hit
TCGATCCGACTCGGTGCCACTTTTTCAACTAGTTCCGCTTACACAGCTGT557580.2072059186019431No Hit
TCGATCCGACTCGGTGCCACTTTTTCAACTAGTTCCGCTTACACAGCTCT545460.2027019268277483No Hit
TCGATCCGACTCGGTGCCACTTTTTCAACTAGTTCCGCTTACACAGCTCA502480.18672985038757556No Hit
TCGATCCGACTCGGTGCCACTTTTTCAACTAGTTCCGCTTACACAGCTTC495690.184206574467874No Hit
CGATCCGACTCGGTGCCACTTTTTCAACTAGTTCCGCTTACACAGCTAAA483280.17959481391360355No Hit
TCGATCCGACTCGGTGCCACTTTTTCAACTAGTTCCGCTTACACAGCTGG471170.17509453830423893No Hit
GGGGGGGGGGGGGGGGGGGGGTGGGGGGGGGGGGGGGGGGGGGGGGGGGG469390.17443306096446443No Hit
CGATCCGACTCGGTGCCACTTTTTCAACTAGTTCCGCTTACACAGCTTAA453930.16868787013698489No Hit
TCGATCCGACTCGGTGCCACTTTTTCAACTAGTTCCGCTTACACAGCTCG450570.1674392387540398No Hit
TCGATCCGACTCGGTGCCACTTTTTCAACTAGTTCCGCTTACACAGCTGC432820.1608430461804459No Hit
CGATCCGACTCGGTGCCACTTTTTCAACTAGTTCCGCTTACACAGCTGAA431280.16027075679659605No Hit
CGATCCGACTCGGTGCCACTTTTTCAACTAGTTCCGCTTACACAGCTATA422620.15705255805364826No Hit
CGATCCGACTCGGTGCCACTTTTTCAACTAGTTCCGCTTACACAGCTAAT422210.1569001952956103No Hit
TCGATCCGACTCGGTGCCACTTTTTCAACTAGTTCCGCTTACACAGCTCC409170.15205431635703767No Hit
CGATCCGACTCGGTGCCACTTTTTCAACTAGTTCCGCTTACACAGCTTTA393840.14635743567235066No Hit
CGATCCGACTCGGTGCCACTTTTTCAACTAGTTCCGCTTACACAGCTAAG388220.14426895103778178No Hit
CGATCCGACTCGGTGCCACTTTTTCAACTAGTTCCGCTTACACAGCTATT388000.1441871954115175No Hit
CGATCCGACTCGGTGCCACTTTTTCAACTAGTTCCGCTTACACAGCTTAC377930.1404450174275124No Hit
CGATCCGACTCGGTGCCACTTTTTCAACTAGTTCCGCTTACACAGCTTGC374260.1390811849348313No Hit
CGATCCGACTCGGTGCCACTTTTTCAACTAGTTCCGCTTACACAGCTAAC371110.13791059301332026No Hit
CGATCCGACTCGGTGCCACTTTTTCAACTAGTTCCGCTTACACAGCTTAT370630.13773221710147096No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN368890.13708560442101725No Hit
CGATCCGACTCGGTGCCACTTTTTCAACTAGTTCCGCTTACACAGCTAGA368110.13679574356426213No Hit
CGATCCGACTCGGTGCCACTTTTTCAACTAGTTCCGCTTACACAGCTATG360170.1338451086890883No Hit
CGATCCGACTCGGTGCCACTTTTTCAACTAGTTCCGCTTACACAGCTTGA348750.12960124845300705No Hit
CGATCCGACTCGGTGCCACTTTTTCAACTAGTTCCGCTTACACAGCTCAA345850.12852356065225085No Hit
CGATCCGACTCGGTGCCACTTTTTCAACTAGTTCCGCTTACACAGCTGAT344620.128066472378137No Hit
CGATCCGACTCGGTGCCACTTTTTCAACTAGTTCCGCTTACACAGCTTGG343580.12767999123579685No Hit
CGATCCGACTCGGTGCCACTTTTTCAACTAGTTCCGCTTACACAGCTGTT342220.12717459281889051No Hit
CGATCCGACTCGGTGCCACTTTTTCAACTAGTTCCGCTTACACAGCTGCC341500.12690702895111655No Hit
CGATCCGACTCGGTGCCACTTTTTCAACTAGTTCCGCTTACACAGCTTAG340120.12639419820454983No Hit
CGATCCGACTCGGTGCCACTTTTTCAACTAGTTCCGCTTACACAGCTGTA339220.1260597433698324No Hit
CGATCCGACTCGGTGCCACTTTTTCAACTAGTTCCGCTTACACAGCTACA338440.12576988251307727No Hit
CGATCCGACTCGGTGCCACTTTTTCAACTAGTTCCGCTTACACAGCTTTT338330.12572900469994516No Hit
CGATCCGACTCGGTGCCACTTTTTCAACTAGTTCCGCTTACACAGCTTTG338030.12561751975503935No Hit
CGATCCGACTCGGTGCCACTTTTTCAACTAGTTCCGCTTACACAGCTGAG335940.12484084130552885No Hit
CGATCCGACTCGGTGCCACTTTTTCAACTAGTTCCGCTTACACAGCTGGA335650.12473307252545322No Hit
CGATCCGACTCGGTGCCACTTTTTCAACTAGTTCCGCTTACACAGCTCTA322400.11980915412544649No Hit
CGATCCGACTCGGTGCCACTTTTTCAACTAGTTCCGCTTACACAGCTACC317770.11808856980906679No Hit
CGATCCGACTCGGTGCCACTTTTTCAACTAGTTCCGCTTACACAGCTTTC311600.11579569610883725No Hit
CGATCCGACTCGGTGCCACTTTTTCAACTAGTTCCGCTTACACAGCTGTC311200.1156470495156295No Hit
CGATCCGACTCGGTGCCACTTTTTCAACTAGTTCCGCTTACACAGCTTCC306770.11400078849585367No Hit
CGATCCGACTCGGTGCCACTTTTTCAACTAGTTCCGCTTACACAGCTCAT305030.11335417581539994No Hit
CGATCCGACTCGGTGCCACTTTTTCAACTAGTTCCGCTTACACAGCTAGT300850.11180081891637897No Hit
CGATCCGACTCGGTGCCACTTTTTCAACTAGTTCCGCTTACACAGCTCTG299690.11136974379607649No Hit
CGATCCGACTCGGTGCCACTTTTTCAACTAGTTCCGCTTACACAGCTGCA297040.11038496011607514No Hit
CGATCCGACTCGGTGCCACTTTTTCAACTAGTTCCGCTTACACAGCTCTT292520.10870525361282757No Hit
CGATCCGACTCGGTGCCACTTTTTCAACTAGTTCCGCTTACACAGCTCAC285700.10617082919863544No Hit
CGATCCGACTCGGTGCCACTTTTTCAACTAGTTCCGCTTACACAGCTTCT285620.10614109987999389No Hit
CGATCCGACTCGGTGCCACTTTTTCAACTAGTTCCGCTTACACAGCTATC285490.10609278973720136No Hit
CGATCCGACTCGGTGCCACTTTTTCAACTAGTTCCGCTTACACAGCTAGG285140.1059627239681446No Hit
CGATCCGACTCGGTGCCACTTTTTCAACTAGTTCCGCTTACACAGCTACT283730.10543874472708727No Hit
CGATCCGACTCGGTGCCACTTTTTCAACTAGTTCCGCTTACACAGCTGGC283610.10539415074912493No Hit
CGATCCGACTCGGTGCCACTTTTTCAACTAGTTCCGCTTACACAGCTCAG275010.10219824899515832No Hit
CGATCCGACTCGGTGCCACTTTTTCAACTAGTTCCGCTTACACAGCTGGG275000.10219453283032813No Hit
CGATCCGACTCGGTGCCACTTTTTCAACTAGTTCCGCTTACACAGCTGTG271220.10078982252451488No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGATC1434750.083.360871
CGCCGTA200050.071.1706284
ACTGGTT1220200.065.324784
ACTGGCT77400.055.369284
GGTCGCC2335600.050.95647484
CGATCCG6811100.048.799381
TCTCTGC38800.046.6257488
GAAACGA66300.043.9939274
ACTCTCT155650.043.934776
TCGCCGT425050.043.83576684
TCGATCC2847100.043.3272671
GTCGCCC27150.042.20620384
GATCCGA8048600.041.3892442
CGAATCC56050.040.963028
ACTGGAT29550.040.4828384
AGTGTAG1812500.040.21454268-69
TCTCTGA130100.040.199538
CTCTCTG172850.040.0029347
GTTCGCC26700.039.93061484
TACTAGC106800.039.4273227
Produced by FastQC (version 0.11.5)