FastQCFastQC Report
Thu 21 Nov 2019
HWV52AFXY_n02_EFS_S6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWV52AFXY_n02_EFS_S6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13966935
Sequences flagged as poor quality0
Sequence length90
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGATCCGACTCGGTGCCACTTTTTCAACTAGTTCCGCTTACACAGCTT292301420.928099114086233No Hit
ATCGATCCGACTCGGTGCCACTTTTTCAACTAGTTCCGCTTACACAGCTA283072920.26736001850084No Hit
ATCGATCCGACTCGGTGCCACTTTTTCAACTAGTTCCGCTTACACAGCTG249580317.86936790355221No Hit
ATCGATCCGACTCGGTGCCACTTTTTCAACTAGTTCCGCTTACACAGCTC228059916.328557410770507No Hit
ATCGATCCGACTCGGTGCCACTTTTTCAACTAGTTCCGCTTACACAGCCT776330.5558341898204581No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG764850.5476147773294571No Hit
ATCGATCAGACTCGGTGCCACTTTTTCAACTAGTTCCGCTTACACAGCTA185550.13284947628094496No Hit
ATCGATCAGACTCGGTGCCACTTTTTCAACTAGTTCCGCTTACACAGCTT180620.12931971116068056No Hit
ATCGATCAGACTCGGTGCCACTTTTTCAACTAGTTCCGCTTACACAGCTG153630.10999550008645419No Hit
ATCGATCAGACTCGGTGCCACTTTTTCAACTAGTTCCGCTTACACAGCTC143770.10293596984592539No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGATC12893800.083.9282761
TCGATCC12656300.083.684332
CGATCCG12635100.083.579453
GATCCGA12728950.083.0230564
ATCCGAC12785950.082.625765
TCCGACT12777800.082.336436
GACTCGG13015950.081.923769
CGACTCG12912850.081.8878568
CCGACTC12938600.081.526437
AGACTCG187450.079.209358
CGATCAG177200.078.228173
GACCTAC9473750.077.0440784
CGAACCG50750.076.314643
TCGATCA201100.074.349872
TCGATCG105550.073.395092
GATCAGA198900.070.872854
TCGAACC59600.068.726252
ATGATCC78650.068.6622541
TCATCCG66750.068.350682
CGACTCT23050.067.585368
Produced by FastQC (version 0.11.5)