FastQCFastQC Report
Thu 21 Nov 2019
HWV52AFXY_n02_EFS_S5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWV52AFXY_n02_EFS_S5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14785011
Sequences flagged as poor quality0
Sequence length90
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCGATCCGACTCGGTGCCACTTTTTCAACTAGTTCCGCTTACACAGCTTG8720605.8982708907014No Hit
TCGATCCGACTCGGTGCCACTTTTTCAACTAGTTCCGCTTACACAGCTAT8593065.812007850383067No Hit
TCGATCCGACTCGGTGCCACTTTTTCAACTAGTTCCGCTTACACAGCTGA8392855.676593679910011No Hit
TCGATCCGACTCGGTGCCACTTTTTCAACTAGTTCCGCTTACACAGCTAA8334325.637006289680812No Hit
TCGATCCGACTCGGTGCCACTTTTTCAACTAGTTCCGCTTACACAGCTTA7958675.3829314026212085No Hit
TCGATCCGACTCGGTGCCACTTTTTCAACTAGTTCCGCTTACACAGCTTT7899165.342681178931825No Hit
TCGATCCGACTCGGTGCCACTTTTTCAACTAGTTCCGCTTACACAGCTAG7701985.209316381299954No Hit
TCGATCCGACTCGGTGCCACTTTTTCAACTAGTTCCGCTTACACAGCTAC7230204.8902229426816115No Hit
TCGATCCGACTCGGTGCCACTTTTTCAACTAGTTCCGCTTACACAGCTGT7062134.776547004259923No Hit
TCGATCCGACTCGGTGCCACTTTTTCAACTAGTTCCGCTTACACAGCTCT6895884.6641020422642905No Hit
TCGATCCGACTCGGTGCCACTTTTTCAACTAGTTCCGCTTACACAGCTTC6234654.216872074021453No Hit
TCGATCCGACTCGGTGCCACTTTTTCAACTAGTTCCGCTTACACAGCTCA6223254.209161562341753No Hit
TCGATCCGACTCGGTGCCACTTTTTCAACTAGTTCCGCTTACACAGCTGG5948934.023622302343908No Hit
TCGATCCGACTCGGTGCCACTTTTTCAACTAGTTCCGCTTACACAGCTCG5726433.873132052455017No Hit
TCGATCCGACTCGGTGCCACTTTTTCAACTAGTTCCGCTTACACAGCTGC5194523.513369046529624No Hit
TCGATCCGACTCGGTGCCACTTTTTCAACTAGTTCCGCTTACACAGCTCC5175483.500491139303177No Hit
TCGATCCGACTCGGTGCCACTTTTTCAACTAGTTCCGCTTACACAGCCTG836210.5655795589195031No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG543150.36736529989730815No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGATCC13411850.083.928851
CGATCCG13470900.083.670932
GATCCGA13572350.083.126493
ATCCGAC13552250.082.7222444
TCCGACT13631500.082.27285
CGACTCG13717350.081.9616857
GACTCGG13795550.081.896348
ACTCGGT13818450.081.721429
CCGACTC13765600.081.4487846
CGATCAG135000.077.451882
CGCTCCG56250.077.072242
ACCTACT9694250.076.785484
TCGATCA147000.075.668321
AGACTCG146800.075.090067
ACCTACC393750.073.73184
TCGATCG103250.073.4623261
ACGATCC102400.073.456561
CCGAATC106100.072.7551356
CGATCGA97450.072.2923052
CGAATCG109250.072.1091847
Produced by FastQC (version 0.11.5)