FastQCFastQC Report
Thu 21 Nov 2019
HWV52AFXY_n02_EFS_S4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWV52AFXY_n02_EFS_S4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27642765
Sequences flagged as poor quality0
Sequence length90
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGATCCGACTCGGTGCCACTTTTTCAACTAGTTCCGCTTACACAGCTTGT6661512.4098566116667417No Hit
CGATCCGACTCGGTGCCACTTTTTCAACTAGTTCCGCTTACACAGCTAAA5260161.9029066014199376No Hit
CGATCCGACTCGGTGCCACTTTTTCAACTAGTTCCGCTTACACAGCTTAA4893151.7701376834046811No Hit
CGATCCGACTCGGTGCCACTTTTTCAACTAGTTCCGCTTACACAGCTATA4677361.6920738573004543No Hit
CGATCCGACTCGGTGCCACTTTTTCAACTAGTTCCGCTTACACAGCTAAT4591701.6610856403113075No Hit
CGATCCGACTCGGTGCCACTTTTTCAACTAGTTCCGCTTACACAGCTGAA4382941.5855649751390644No Hit
CGATCCGACTCGGTGCCACTTTTTCAACTAGTTCCGCTTACACAGCTTTA4281691.54893694606889No Hit
CGATCCGACTCGGTGCCACTTTTTCAACTAGTTCCGCTTACACAGCTAAG4266011.5432645757398002No Hit
CGATCCGACTCGGTGCCACTTTTTCAACTAGTTCCGCTTACACAGCTATT4256401.539788078363362No Hit
CGATCCGACTCGGTGCCACTTTTTCAACTAGTTCCGCTTACACAGCTTAT4084081.4774498860732637No Hit
CGATCCGACTCGGTGCCACTTTTTCAACTAGTTCCGCTTACACAGCTTGC4063861.4701351330085828No Hit
CGATCCGACTCGGTGCCACTTTTTCAACTAGTTCCGCTTACACAGCTAGA4006771.4494823509876817No Hit
CGATCCGACTCGGTGCCACTTTTTCAACTAGTTCCGCTTACACAGCTATG3921071.4184796636660624No Hit
CGATCCGACTCGGTGCCACTTTTTCAACTAGTTCCGCTTACACAGCTAAC3890471.407409859324854No Hit
CGATCCGACTCGGTGCCACTTTTTCAACTAGTTCCGCTTACACAGCTTAC3779891.3674066252055466No Hit
CGATCCGACTCGGTGCCACTTTTTCAACTAGTTCCGCTTACACAGCTTGA3770961.3641761234811351No Hit
CGATCCGACTCGGTGCCACTTTTTCAACTAGTTCCGCTTACACAGCTTGG3764051.3616763735465682No Hit
CGATCCGACTCGGTGCCACTTTTTCAACTAGTTCCGCTTACACAGCTTTG3762501.3611156481632718No Hit
CGATCCGACTCGGTGCCACTTTTTCAACTAGTTCCGCTTACACAGCTCAA3751661.3571941880633143No Hit
CGATCCGACTCGGTGCCACTTTTTCAACTAGTTCCGCTTACACAGCTTAG3740011.3529797037307953No Hit
CGATCCGACTCGGTGCCACTTTTTCAACTAGTTCCGCTTACACAGCTGTT3736051.3515471408160509No Hit
CGATCCGACTCGGTGCCACTTTTTCAACTAGTTCCGCTTACACAGCTGAT3725451.3477125027109265No Hit
CGATCCGACTCGGTGCCACTTTTTCAACTAGTTCCGCTTACACAGCTGTA3708941.3417398729830392No Hit
CGATCCGACTCGGTGCCACTTTTTCAACTAGTTCCGCTTACACAGCTTTT3700431.3386613097495856No Hit
CGATCCGACTCGGTGCCACTTTTTCAACTAGTTCCGCTTACACAGCTGGA3664401.3256271577752803No Hit
CGATCCGACTCGGTGCCACTTTTTCAACTAGTTCCGCTTACACAGCTACA3663731.3253847797063716No Hit
CGATCCGACTCGGTGCCACTTTTTCAACTAGTTCCGCTTACACAGCTGAC3565841.2899722585638593No Hit
CGATCCGACTCGGTGCCACTTTTTCAACTAGTTCCGCTTACACAGCTGAG3551841.2849076421986005No Hit
CGATCCGACTCGGTGCCACTTTTTCAACTAGTTCCGCTTACACAGCTCTA3514381.2713561758384155No Hit
CGATCCGACTCGGTGCCACTTTTTCAACTAGTTCCGCTTACACAGCTACC3462481.252580919455778No Hit
CGATCCGACTCGGTGCCACTTTTTCAACTAGTTCCGCTTACACAGCTTTC3410771.2338743971523833No Hit
CGATCCGACTCGGTGCCACTTTTTCAACTAGTTCCGCTTACACAGCTGTC3394921.2281405279102868No Hit
CGATCCGACTCGGTGCCACTTTTTCAACTAGTTCCGCTTACACAGCTCAT3354521.2135254921133976No Hit
CGATCCGACTCGGTGCCACTTTTTCAACTAGTTCCGCTTACACAGCTTCC3314541.199062394807466No Hit
CGATCCGACTCGGTGCCACTTTTTCAACTAGTTCCGCTTACACAGCTAGT3249271.1754504297960064No Hit
CGATCCGACTCGGTGCCACTTTTTCAACTAGTTCCGCTTACACAGCTGCA3244951.173887633889012No Hit
CGATCCGACTCGGTGCCACTTTTTCAACTAGTTCCGCTTACACAGCTCTG3222131.1656323092136405No Hit
CGATCCGACTCGGTGCCACTTTTTCAACTAGTTCCGCTTACACAGCTCTT3190461.1541754234788018No Hit
CGATCCGACTCGGTGCCACTTTTTCAACTAGTTCCGCTTACACAGCTTCT3130941.132643568760216No Hit
CGATCCGACTCGGTGCCACTTTTTCAACTAGTTCCGCTTACACAGCTAGG3099161.121146889611079No Hit
CGATCCGACTCGGTGCCACTTTTTCAACTAGTTCCGCTTACACAGCTATC3096861.1203148454939293No Hit
CGATCCGACTCGGTGCCACTTTTTCAACTAGTTCCGCTTACACAGCTACT3096281.120105025673083No Hit
CGATCCGACTCGGTGCCACTTTTTCAACTAGTTCCGCTTACACAGCTGGG2984341.0796098002497219No Hit
CGATCCGACTCGGTGCCACTTTTTCAACTAGTTCCGCTTACACAGCTCAG2952161.0679684177758628No Hit
CGATCCGACTCGGTGCCACTTTTTCAACTAGTTCCGCTTACACAGCTGTG2940421.0637213751952816No Hit
CGATCCGACTCGGTGCCACTTTTTCAACTAGTTCCGCTTACACAGCTACG2937311.0625963068455706No Hit
CGATCCGACTCGGTGCCACTTTTTCAACTAGTTCCGCTTACACAGCTGCC2922491.0572350486646325No Hit
CGATCCGACTCGGTGCCACTTTTTCAACTAGTTCCGCTTACACAGCTGGT2907361.051761645407035No Hit
CGATCCGACTCGGTGCCACTTTTTCAACTAGTTCCGCTTACACAGCTGCT2896511.0478365677239596No Hit
CGATCCGACTCGGTGCCACTTTTTCAACTAGTTCCGCTTACACAGCTCGT2865371.0365714138943771No Hit
CGATCCGACTCGGTGCCACTTTTTCAACTAGTTCCGCTTACACAGCTGGC2862921.0356851060304568No Hit
CGATCCGACTCGGTGCCACTTTTTCAACTAGTTCCGCTTACACAGCTCAC2785431.00765245444875No Hit
CGATCCGACTCGGTGCCACTTTTTCAACTAGTTCCGCTTACACAGCTAGC2760520.9986410549017076No Hit
CGATCCGACTCGGTGCCACTTTTTCAACTAGTTCCGCTTACACAGCTTCA2752090.9955914323331982No Hit
CGATCCGACTCGGTGCCACTTTTTCAACTAGTTCCGCTTACACAGCTCGA2603110.9416966790406097No Hit
CGATCCGACTCGGTGCCACTTTTTCAACTAGTTCCGCTTACACAGCTTCG2574980.9315204177295578No Hit
CGATCCGACTCGGTGCCACTTTTTCAACTAGTTCCGCTTACACAGCTCGG2544070.9203384683116902No Hit
CGATCCGACTCGGTGCCACTTTTTCAACTAGTTCCGCTTACACAGCTCCG2512170.9087983781651366No Hit
CGATCCGACTCGGTGCCACTTTTTCAACTAGTTCCGCTTACACAGCTCTC2494140.9022758758033069No Hit
CGATCCGACTCGGTGCCACTTTTTCAACTAGTTCCGCTTACACAGCTCCC2468700.8930727443510083No Hit
CGATCCGACTCGGTGCCACTTTTTCAACTAGTTCCGCTTACACAGCTCGC2444180.8842024305455696No Hit
CGATCCGACTCGGTGCCACTTTTTCAACTAGTTCCGCTTACACAGCTCCA2443130.8838225843181752No Hit
CGATCCGACTCGGTGCCACTTTTTCAACTAGTTCCGCTTACACAGCTCCT2369970.8573563462265805No Hit
CGATCCGACTCGGTGCCACTTTTTCAACTAGTTCCGCTTACACAGCTGCG2033000.7354546478979219No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG771830.27921591779982935No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATCCG25401200.083.947121
GATCCGA25517700.083.527952
ATCCGAC25556200.083.1313
TCCGACT25643300.082.775574
GACTCGG25980650.082.5220957
CGACTCG25881250.082.507626
ACTCGGT25939600.082.3309868
CTCGGTG25961550.082.139569
CCGACTC25948700.082.008925
CCTACTG18911000.077.8741384
AGACTCG220500.074.9901356
CGATCAG198500.074.689961
CCTACCT668150.074.5303184
CCTCGGT66950.072.1294868
CTACTGG1443150.069.1256184
CCTACTT162400.068.8104384
GCCTCGG76150.068.325857
CGCCTCG76200.067.850086
CGAATCG131550.067.780766
CGATACG159600.067.748711
Produced by FastQC (version 0.11.5)