FastQCFastQC Report
Thu 21 Nov 2019
HWV52AFXY_n02_EFS_S1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWV52AFXY_n02_EFS_S1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18053575
Sequences flagged as poor quality0
Sequence length90
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCGACTCGGTGCCACTTTTTCAACTAGTTCCGCTTACACAGCTTGTCTC1683380.9324358194983543No Hit
TCCGACTCGGTGCCACTTTTTCAACTAGTTCCGCTTACACAGCCTGTCTC644250.3568545288121605No Hit
TCCGACTCGGTGCCACTTTTTCAACTAGTTCCGCTTACACAGCTCTGTCT549720.30449370830985No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG407680.22581677036265668No Hit
TCCGACTCGGTGCCACTTTTTCAACTAGTTCCGCTTACACAGCTGTCTCT186220.10314854537120763No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGACT16801150.083.721061
GACTCGG16977450.083.4053654
CGACTCG16972000.083.400243
ACTCGGT16944800.083.1698465
CTCGGTG16965600.083.055326
TCGGTGC16971350.082.915767
CGGTGCC16929950.082.8157658
CCGACTC17103000.082.8086242
GGTGCCA16987900.082.6359949
ACTGGTT11321450.078.44527484
ACTGGCT633650.075.2800684
CGGAGCC125450.074.307178
TCGGAGC138500.073.5550167
ACTCGGA135250.072.2938165
GGAGCCA130500.071.561379
AGACTCG124200.069.297753
CTGGTTC760700.069.21010684
CTCGGAG145700.068.892536
GGTGTAG305250.065.29220684
CGGTGCA107150.065.287678
Produced by FastQC (version 0.11.5)