FastQCFastQC Report
Thu 21 Nov 2019
HWV52AFXY_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWV52AFXY_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26909463
Sequences flagged as poor quality0
Sequence length60
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG374895213.93172357248452No Hit
GCTTTCTTGTACAAAGTGGTTTAATGAGTTTAAACCTCGAGCGTAACTATAACGGTCCTA6294792.3392477211455316No Hit
CCCAGCTTTCTTGTACAAAGTGGTTTAATGAGTTTAAACCTCGAGCGTAACTATAACGGT855700.31799222451967923No Hit
AGCTTTCTTGTACAAAGTGGTTTAATGAGTTTAAACCTCGAGCGTAACTATAACGGTCCT817700.30387079816494295No Hit
CCTAGAACCCAGCTTTCTTGTACAAAGTGGTTTAATGAGTTTAAACCTCGAGCGTAACTA547290.20338198499167373No Hit
GCTAGAACCCAGCTTTCTTGTACAAAGTGGTTTAATGAGTTTAAACCTCGAGCGTAACTA400380.14878780747129736No Hit
GTTTAAACCTCGAGCGTAACTATAACGGTCCTAAGGTAGCGAACCAGTAGGTCCACTATG325320.12089427425586309No Hit
GCTGTGTAAGCGGAACTAGTTGAAAAAGTGGCACCGAGTCGGATCGAGATCGGAAGAGCA297320.11048901273132058No Hit
GTTTAATGAGTTTAAACCTCGAGCGTAACTATAACGGTCCTAAGGTAGCGAACCAGTAGG295390.10977179291909318No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAAGGT2276750.034.59752
AAGGTAG2282900.034.569434
CCTAAGG2277050.034.5530361
TAGCGAA2300350.034.068648
GGTAGCG2320600.033.8214386
AGGTAGC2337950.033.6710325
TAAGGTA2344650.033.6291163
GTAGCGA2342450.033.5336157
AGCGAAC2362100.033.2283069
GTATACG3600.030.7456656
CGTATAC5150.028.8309925
GCGAACC2904750.026.93062210
GTATACT6800.026.5991886
GAACCAG2941400.026.5591912
GGTATAC4900.026.4453475
CGAACCA2971950.026.33530611
AACCAGT3011750.025.98839813
ACCAGTA3010250.025.97223514
GGTCCTA1587000.025.23513854
ACGGTCC1674500.023.92095252
Produced by FastQC (version 0.11.5)