FastQCFastQC Report
Thu 21 Nov 2019
HWV52AFXY_n01_EFS_S5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWV52AFXY_n01_EFS_S5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14785011
Sequences flagged as poor quality0
Sequence length60
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTTTCTTGTACAAAGTGGTTTAATGAGTTTAAACCTCGAGCGTAACTATAACGGTCCTA8205405.5498098716328315No Hit
GCTGTGTAAGCGGAACTAGTTGAAAAAGTGGCACCGAGTCGGATCGAAGATCGGAAGAGC2171121.4684601857922188No Hit
CCCAGCTTTCTTGTACAAAGTGGTTTAATGAGTTTAAACCTCGAGCGTAACTATAACGGT1151860.7790728055596307No Hit
AGCTTTCTTGTACAAAGTGGTTTAATGAGTTTAAACCTCGAGCGTAACTATAACGGTCCT1090910.7378486225001795No Hit
CCTAGAACCCAGCTTTCTTGTACAAAGTGGTTTAATGAGTTTAAACCTCGAGCGTAACTA804110.5438683812950832No Hit
GCTAGAACCCAGCTTTCTTGTACAAAGTGGTTTAATGAGTTTAAACCTCGAGCGTAACTA563760.38130509338139823No Hit
GTTTAAACCTCGAGCGTAACTATAACGGTCCTAAGGTAGCGAACCAGTAGGTCCACTATG448770.30353037951747214No Hit
GTTTAATGAGTTTAAACCTCGAGCGTAACTATAACGGTCCTAAGGTAGCGAACCAGTAGG406100.27467006957248796No Hit
AGCTGTGTAAGCGGAACTAGTTGAAAAAGTGGCACCGAGTCGGATCGAAGATCGGAAGAG405850.2745009794040735No Hit
GTACAAAGTGGTTTAATGAGTTTAAACCTCGAGCGTAACTATAACGGTCCTAAGGTAGCG360400.24376038678632028No Hit
CTTTCTTGTACAAAGTGGTTTAATGAGTTTAAACCTCGAGCGTAACTATAACGGTCCTAA348600.23577933083715666No Hit
GTAGAACCCAGCTTTCTTGTACAAAGTGGTTTAATGAGTTTAAACCTCGAGCGTAACTAT299690.20269853028854695No Hit
ACTAGAACCCAGCTTTCTTGTACAAAGTGGTTTAATGAGTTTAAACCTCGAGCGTAACTA254090.17185648356974506No Hit
AGTAGAACCCAGCTTTCTTGTACAAAGTGGTTTAATGAGTTTAAACCTCGAGCGTAACTA239790.16218452593643656No Hit
CTAGAACCCAGCTTTCTTGTACAAAGTGGTTTAATGAGTTTAAACCTCGAGCGTAACTAT233930.15822105238880105No Hit
GGTAGAACCCAGCTTTCTTGTACAAAGTGGTTTAATGAGTTTAAACCTCGAGCGTAACTA230450.15586731724447145No Hit
AGAACCCAGCTTTCTTGTACAAAGTGGTTTAATGAGTTTAAACCTCGAGCGTAACTATAA197290.13343919730597426No Hit
TCTAGAACCCAGCTTTCTTGTACAAAGTGGTTTAATGAGTTTAAACCTCGAGCGTAACTA190750.12901579850025136No Hit
CTTAGAACCCAGCTTTCTTGTACAAAGTGGTTTAATGAGTTTAAACCTCGAGCGTAACTA172030.11635432668937479No Hit
CATAGAACCCAGCTTTCTTGTACAAAGTGGTTTAATGAGTTTAAACCTCGAGCGTAACTA150080.1015082099025831No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAAGAGC375400.034.9903954
GTAGCGA2706650.034.0331277
TAGCGAA2699350.033.9969028
AGCGAAC2707300.033.908049
TAAGGTA2734950.033.871733
CTAAGGT2740250.033.8318332
AAGGTAG2739000.033.799964
CCTAAGG2744800.033.799331
GGTAGCG2737900.033.685116
AGGTAGC2752350.033.5543675
CGGAAGA414350.031.53829452
GGAAGAG418550.031.35083253
TCGGAAG441200.029.63121251
ATCGGAA497000.026.40762950
GCGAACC3477000.026.34050610
CGAACCA3494300.026.18382511
AACCAGT3517550.025.94483413
GAACCAG3528400.025.87729812
ACCAGTA3565050.025.56355514
GGTCCTA1981650.025.09160854
Produced by FastQC (version 0.11.5)