FastQCFastQC Report
Thu 21 Nov 2019
HWV52AFXY_n01_EFS_S4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWV52AFXY_n01_EFS_S4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27642765
Sequences flagged as poor quality0
Sequence length60
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTTTCTTGTACAAAGTGGTTTAATGAGTTTAAACCTCGAGCGTAACTATAACGGTCCTA14347515.19033099619376No Hit
GCTGTGTAAGCGGAACTAGTTGAAAAAGTGGCACCGAGTCGGATCGAGATCGGAAGAGCA2861741.0352582312225278No Hit
CCCAGCTTTCTTGTACAAAGTGGTTTAATGAGTTTAAACCTCGAGCGTAACTATAACGGT2028510.7338303530779211No Hit
AGCTTTCTTGTACAAAGTGGTTTAATGAGTTTAAACCTCGAGCGTAACTATAACGGTCCT1921820.6952343587915318No Hit
CCTAGAACCCAGCTTTCTTGTACAAAGTGGTTTAATGAGTTTAAACCTCGAGCGTAACTA1412610.5110234088377194No Hit
GCTAGAACCCAGCTTTCTTGTACAAAGTGGTTTAATGAGTTTAAACCTCGAGCGTAACTA1012520.3662875258679803No Hit
GTTTAAACCTCGAGCGTAACTATAACGGTCCTAAGGTAGCGAACCAGTAGGTCCACTATG765740.27701280968094183No Hit
GTTTAATGAGTTTAAACCTCGAGCGTAACTATAACGGTCCTAAGGTAGCGAACCAGTAGG719080.26013316685215826No Hit
CTTTCTTGTACAAAGTGGTTTAATGAGTTTAAACCTCGAGCGTAACTATAACGGTCCTAA631530.22846122665370125No Hit
GTACAAAGTGGTTTAATGAGTTTAAACCTCGAGCGTAACTATAACGGTCCTAAGGTAGCG628410.22733254072087217No Hit
AGCTGTGTAAGCGGAACTAGTTGAAAAAGTGGCACCGAGTCGGATCGAGATCGGAAGAGC607980.21994181841071253No Hit
ACTAGAACCCAGCTTTCTTGTACAAAGTGGTTTAATGAGTTTAAACCTCGAGCGTAACTA579210.2095340317801059No Hit
GTAGAACCCAGCTTTCTTGTACAAAGTGGTTTAATGAGTTTAAACCTCGAGCGTAACTAT506130.1830967343534556No Hit
CTAGAACCCAGCTTTCTTGTACAAAGTGGTTTAATGAGTTTAAACCTCGAGCGTAACTAT436560.15792920860123796No Hit
GGTAGAACCCAGCTTTCTTGTACAAAGTGGTTTAATGAGTTTAAACCTCGAGCGTAACTA424670.15362790227388615No Hit
TCTAGAACCCAGCTTTCTTGTACAAAGTGGTTTAATGAGTTTAAACCTCGAGCGTAACTA360550.13043195932100135No Hit
CTGTGTAAGCGGAACTAGTTGAAAAAGTGGCACCGAGTCGGATCGAGATCGGAAGAGCAC323400.11699263803747564No Hit
GTTAGAACCCAGCTTTCTTGTACAAAGTGGTTTAATGAGTTTAAACCTCGAGCGTAACTA300990.1088856342699437No Hit
AGTAGAACCCAGCTTTCTTGTACAAAGTGGTTTAATGAGTTTAAACCTCGAGCGTAACTA299310.10827788030611264No Hit
AGAACCCAGCTTTCTTGTACAAAGTGGTTTAATGAGTTTAAACCTCGAGCGTAACTATAA297620.10766650875916356No Hit
CATAGAACCCAGCTTTCTTGTACAAAGTGGTTTAATGAGTTTAAACCTCGAGCGTAACTA280220.10137191413377063No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGAGCA502000.035.26107854
GTAGCGA4875500.033.730497
TAAGGTA4898650.033.684693
TAGCGAA4877350.033.6523748
AGCGAAC4889950.033.5844359
CTAAGGT4917600.033.580692
CCTAAGG4926500.033.5378841
AAGGTAG4920700.033.5370374
GGTAGCG4918700.033.4380876
AGGTAGC4954650.033.2690935
CGGAAGA628550.028.44954151
GAAGAGC637400.028.10960253
GGAAGAG681700.026.28687152
GCGAACC6266600.026.15747310
CGAACCA6282800.026.06295211
TCGGAAG687750.025.97711450
AACCAGT6341900.025.8223413
GAACCAG6354500.025.7511712
ACCAGTA6431650.025.4485714
GGTCCTA3587450.024.68962954
Produced by FastQC (version 0.11.5)