FastQCFastQC Report
Thu 21 Nov 2019
HWV52AFXY_n01_EFS_S3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWV52AFXY_n01_EFS_S3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14521772
Sequences flagged as poor quality0
Sequence length60
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTTTCTTGTACAAAGTGGTTTAATGAGTTTAAACCTCGAGCGTAACTATAACGGTCCTA8031425.530606044496498No Hit
GCTGTGTAAGCGGAACTAGTTGAAAAAGTGGCACCGAGTCGGATCAGATCGGAAGAGCAC2176121.4985223566380192No Hit
CCCAGCTTTCTTGTACAAAGTGGTTTAATGAGTTTAAACCTCGAGCGTAACTATAACGGT1093790.7532069777710324No Hit
AGCTTTCTTGTACAAAGTGGTTTAATGAGTTTAAACCTCGAGCGTAACTATAACGGTCCT1080000.7437108914807367No Hit
CCTAGAACCCAGCTTTCTTGTACAAAGTGGTTTAATGAGTTTAAACCTCGAGCGTAACTA764130.5261961143584957No Hit
GCTAGAACCCAGCTTTCTTGTACAAAGTGGTTTAATGAGTTTAAACCTCGAGCGTAACTA615790.4240460461712248No Hit
GTTTAAACCTCGAGCGTAACTATAACGGTCCTAAGGTAGCGAACCAGTAGGTCCACTATG443800.30561008670291756No Hit
AGCTGTGTAAGCGGAACTAGTTGAAAAAGTGGCACCGAGTCGGATCAGATCGGAAGAGCA439270.30249063268587334No Hit
GTTTAATGAGTTTAAACCTCGAGCGTAACTATAACGGTCCTAAGGTAGCGAACCAGTAGG408480.2812879860667142No Hit
GTACAAAGTGGTTTAATGAGTTTAAACCTCGAGCGTAACTATAACGGTCCTAAGGTAGCG362480.24961141105920132No Hit
ACTAGAACCCAGCTTTCTTGTACAAAGTGGTTTAATGAGTTTAAACCTCGAGCGTAACTA349400.24060424581793463No Hit
CTTTCTTGTACAAAGTGGTTTAATGAGTTTAAACCTCGAGCGTAACTATAACGGTCCTAA329200.22669409766246157No Hit
GTAGAACCCAGCTTTCTTGTACAAAGTGGTTTAATGAGTTTAAACCTCGAGCGTAACTAT320640.22079950022628092No Hit
CTAGAACCCAGCTTTCTTGTACAAAGTGGTTTAATGAGTTTAAACCTCGAGCGTAACTAT282710.19468009826899912No Hit
GGTAGAACCCAGCTTTCTTGTACAAAGTGGTTTAATGAGTTTAAACCTCGAGCGTAACTA260640.17948222847735112No Hit
TCTAGAACCCAGCTTTCTTGTACAAAGTGGTTTAATGAGTTTAAACCTCGAGCGTAACTA224020.15426492028658761No Hit
GCCTAGAACCCAGCTTTCTTGTACAAAGTGGTTTAATGAGTTTAAACCTCGAGCGTAACT162790.11210064446680473No Hit
AGAACCCAGCTTTCTTGTACAAAGTGGTTTAATGAGTTTAAACCTCGAGCGTAACTATAA152870.10526952220431501No Hit
AGTAGAACCCAGCTTTCTTGTACAAAGTGGTTTAATGAGTTTAAACCTCGAGCGTAACTA152820.10523509114452423No Hit
CTGTGTAAGCGGAACTAGTTGAAAAAGTGGCACCGAGTCGGATCAGATCGGAAGAGCACA152690.10514557038906823No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGAGCAC329650.039.67453854
TAGCGAA2632400.033.8728528
GTAGCGA2648650.033.81417
AGCGAAC2638100.033.7730529
TAAGGTA2676200.033.6588133
CCTAAGG2676750.033.6230621
AAGGTAG2684650.033.580034
CTAAGGT2681950.033.5735632
GGTAGCG2670500.033.4949656
AGGTAGC2694850.033.279485
AAGAGCA401700.032.82053853
GAAGAGC460200.028.60736552
GCGAACC3333500.026.65639510
CGAACCA3343350.026.52698711
AACCAGT3371150.026.29141213
GAACCAG3379300.026.22161312
CGGAAGA500650.026.1396450
ACCAGTA3411350.025.92460414
GGAAGAG522000.025.1843151
GGTCCTA1956700.025.13837654
Produced by FastQC (version 0.11.5)