FastQCFastQC Report
Thu 21 Nov 2019
HWV52AFXY_n01_EFS_S2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWV52AFXY_n01_EFS_S2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19060032
Sequences flagged as poor quality0
Sequence length60
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTTTCTTGTACAAAGTGGTTTAATGAGTTTAAACCTCGAGCGTAACTATAACGGTCCTA10248665.3770423890159265No Hit
GCTGTGTAAGCGGAACTAGTTGAAAAAGTGGCACCGAGTCGGATAGATCGGAAGAGCACA2431541.2757271341412229No Hit
CCCAGCTTTCTTGTACAAAGTGGTTTAATGAGTTTAAACCTCGAGCGTAACTATAACGGT1555150.8159220299315342No Hit
AGCTTTCTTGTACAAAGTGGTTTAATGAGTTTAAACCTCGAGCGTAACTATAACGGTCCT1361710.7144321688442077No Hit
CCTAGAACCCAGCTTTCTTGTACAAAGTGGTTTAATGAGTTTAAACCTCGAGCGTAACTA916300.48074420861413036No Hit
GCTAGAACCCAGCTTTCTTGTACAAAGTGGTTTAATGAGTTTAAACCTCGAGCGTAACTA645860.33885567453401966No Hit
GTTTAAACCTCGAGCGTAACTATAACGGTCCTAAGGTAGCGAACCAGTAGGTCCACTATG601890.31578645828086754No Hit
GTTTAATGAGTTTAAACCTCGAGCGTAACTATAACGGTCCTAAGGTAGCGAACCAGTAGG514900.27014645096083784No Hit
AGCTGTGTAAGCGGAACTAGTTGAAAAAGTGGCACCGAGTCGGATAGATCGGAAGAGCAC497560.26104887966609924No Hit
GTACAAAGTGGTTTAATGAGTTTAAACCTCGAGCGTAACTATAACGGTCCTAAGGTAGCG477990.2507813208288423No Hit
ACTAGAACCCAGCTTTCTTGTACAAAGTGGTTTAATGAGTTTAAACCTCGAGCGTAACTA429800.22549804743244922No Hit
GTAGAACCCAGCTTTCTTGTACAAAGTGGTTTAATGAGTTTAAACCTCGAGCGTAACTAT411570.21593353043688487No Hit
CTAGAACCCAGCTTTCTTGTACAAAGTGGTTTAATGAGTTTAAACCTCGAGCGTAACTAT298810.15677308411654292No Hit
AGTAGAACCCAGCTTTCTTGTACAAAGTGGTTTAATGAGTTTAAACCTCGAGCGTAACTA295030.15478987653326082No Hit
CTTTCTTGTACAAAGTGGTTTAATGAGTTTAAACCTCGAGCGTAACTATAACGGTCCTAA293620.15405010862521112No Hit
CATAGAACCCAGCTTTCTTGTACAAAGTGGTTTAATGAGTTTAAACCTCGAGCGTAACTA279640.14671538851561214No Hit
GGTAGAACCCAGCTTTCTTGTACAAAGTGGTTTAATGAGTTTAAACCTCGAGCGTAACTA271520.1424551648181913No Hit
TCTAGAACCCAGCTTTCTTGTACAAAGTGGTTTAATGAGTTTAAACCTCGAGCGTAACTA231100.12124848478743372No Hit
AGAACCCAGCTTTCTTGTACAAAGTGGTTTAATGAGTTTAAACCTCGAGCGTAACTATAA195110.10236604009898828No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCACA383200.038.74525554
TAGCGAA3361600.034.747288
GTAGCGA3371150.034.7211077
AGAGCAC433200.034.67215753
AGCGAAC3380900.034.572889
TAAGGTA3407350.034.517143
AAGGTAG3410950.034.506044
CTAAGGT3416450.034.4528582
CCTAAGG3422150.034.4123041
GGTAGCG3400750.034.4046066
AGGTAGC3422850.034.250315
AAGAGCA495350.030.4745752
GCGAACC4406150.026.45410710
CGAACCA4418100.026.35627611
GAAGAGC574150.026.33907151
AACCAGT4455850.026.12517713
GAACCAG4465700.026.03006712
ACCAGTA4512750.025.76106814
GGTCCTA2424300.025.59650254
CACACGT36950.025.13646954
Produced by FastQC (version 0.11.5)