FastQCFastQC Report
Thu 21 Nov 2019
HWV52AFXY_n01_EFS_S1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWV52AFXY_n01_EFS_S1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18053575
Sequences flagged as poor quality0
Sequence length60
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTTTCTTGTACAAAGTGGTTTAATGAGTTTAAACCTCGAGCGTAACTATAACGGTCCTA10795225.979546987231061No Hit
GCTGTGTAAGCGGAACTAGTTGAAAAAGTGGCACCGAGTCGGAAGATCGGAAGAGCACAC2290171.2685409953430276No Hit
CCCAGCTTTCTTGTACAAAGTGGTTTAATGAGTTTAAACCTCGAGCGTAACTATAACGGT1540480.8532825216058316No Hit
AGCTTTCTTGTACAAAGTGGTTTAATGAGTTTAAACCTCGAGCGTAACTATAACGGTCCT1483490.821715366624062No Hit
CCTAGAACCCAGCTTTCTTGTACAAAGTGGTTTAATGAGTTTAAACCTCGAGCGTAACTA878430.48656844973917907No Hit
GCTAGAACCCAGCTTTCTTGTACAAAGTGGTTTAATGAGTTTAAACCTCGAGCGTAACTA698440.3868707444370436No Hit
GTTTAATGAGTTTAAACCTCGAGCGTAACTATAACGGTCCTAAGGTAGCGAACCAGTAGG536830.29735384819904087No Hit
AGCTGTGTAAGCGGAACTAGTTGAAAAAGTGGCACCGAGTCGGAAGATCGGAAGAGCACA520950.28855780641784246No Hit
GTTTAAACCTCGAGCGTAACTATAACGGTCCTAAGGTAGCGAACCAGTAGGTCCACTATG514450.2849574114822134No Hit
ACTAGAACCCAGCTTTCTTGTACAAAGTGGTTTAATGAGTTTAAACCTCGAGCGTAACTA425870.23589233711328642No Hit
GTACAAAGTGGTTTAATGAGTTTAAACCTCGAGCGTAACTATAACGGTCCTAAGGTAGCG401360.22231607867139888No Hit
CTTTCTTGTACAAAGTGGTTTAATGAGTTTAAACCTCGAGCGTAACTATAACGGTCCTAA311970.17280233970280123No Hit
GGTAGAACCCAGCTTTCTTGTACAAAGTGGTTTAATGAGTTTAAACCTCGAGCGTAACTA286230.15854477575771006No Hit
GTAGAACCCAGCTTTCTTGTACAAAGTGGTTTAATGAGTTTAAACCTCGAGCGTAACTAT274500.15204744766618244No Hit
AGAACCCAGCTTTCTTGTACAAAGTGGTTTAATGAGTTTAAACCTCGAGCGTAACTATAA251540.13932974493971417No Hit
TCTAGAACCCAGCTTTCTTGTACAAAGTGGTTTAATGAGTTTAAACCTCGAGCGTAACTA241090.13354141769704894No Hit
CTAGAACCCAGCTTTCTTGTACAAAGTGGTTTAATGAGTTTAAACCTCGAGCGTAACTAT238670.13220096296716854No Hit
GATAGAACCCAGCTTTCTTGTACAAAGTGGTTTAATGAGTTTAAACCTCGAGCGTAACTA214770.11896258774231697No Hit
AGTAGAACCCAGCTTTCTTGTACAAAGTGGTTTAATGAGTTTAAACCTCGAGCGTAACTA211660.11723993724234674No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC336850.041.03075454
GAGCACA405100.034.3179753
TAGCGAA3248000.032.789668
GTAGCGA3258800.032.7348447
AGCGAAC3257300.032.7250489
TAAGGTA3293750.032.6022643
CCTAAGG3302300.032.549841
CTAAGGT3305950.032.5456542
AAGGTAG3293400.032.5294884
GGTAGCG3286950.032.445466
AGGTAGC3302900.032.3517235
AGAGCAC434350.031.91990352
AAGAGCA511700.027.25837351
ACACGTC27500.026.01801354
GCGAACC4098250.025.92167710
CGAACCA4122950.025.77424211
GGTCCTA2555350.025.56654754
AACCAGT4153850.025.5442313
GAACCAG4159350.025.50266612
GCACACG63200.025.41913854
Produced by FastQC (version 0.11.5)