FastQCFastQC Report
Tue 8 May 2018
HWTTCBGX5_n01_mb99.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWTTCBGX5_n01_mb99.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29213597
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG9075513.1066047772206895TruSeq Adapter, Index 10 (100% over 63bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG1194850.064.9180943
CTCGTAT1193900.064.9112942
TCTCGTA1186900.064.8841941
CGTATGC1209900.064.8533444
ATGCCGT1202800.064.6676147
TATGCCG1215000.064.60210446
TATCTCG1198850.064.5940339
ATCTCGT1201050.064.3525340
CCGTCTT1194750.064.2945750
TGCCGTC1204400.064.2538448
GCCGTCT1189400.064.2031249
CGTCTTC1196850.063.58173451
GTATGCC1250200.063.04258745
GCTTATC1265800.062.8991936
TAGCTTA1316150.061.7921534
ACTAGCT1332750.061.48810232
AGCTTAT1310800.061.38493735
GTCACTA1349250.061.0634429
CGTCTGA1362500.061.05037716
CACTAGC1353100.060.77027531
Produced by FastQC (version 0.11.2)