FastQCFastQC Report
Tue 8 May 2018
HWTTCBGX5_n01_mb98.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWTTCBGX5_n01_mb98.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34237907
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG15549724.5416678069719625TruSeq Adapter, Index 9 (100% over 63bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT2031150.062.93284242
TCTCGTA2014200.062.8695641
TCGTATG2064650.062.8378343
CGTATGC2068900.062.74347344
GATCTCG1993300.062.6674439
ATGCCGT2066100.062.643347
TATGCCG2071550.062.57538246
ATCTCGT1999800.062.51314540
CGATCAG2072900.062.3947833
TGCCGTC2071900.062.39446348
GCCGTCT2068750.062.3627249
ACGATCA2080700.062.3617732
CCGTCTT2067950.062.36051650
CACGATC2089950.062.0765831
CGTCTTC2080950.062.04039851
GTCACGA2096050.062.032229
TCACGAT2099050.061.8963630
AGTCACG2120650.061.47773728
GTATGCC2110250.061.47340845
CGTCTGA2174450.061.0138216
Produced by FastQC (version 0.11.2)