FastQCFastQC Report
Tue 8 May 2018
HWTTCBGX5_n01_mb97.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWTTCBGX5_n01_mb97.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23021947
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG6818552.9617607928643044TruSeq Adapter, Index 8 (100% over 63bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATCTCG941600.064.8066639
TCTCGTA945350.064.0533141
CTCGTAT949950.063.89272342
TCGTATG959100.063.83072743
CGTATGC976400.063.76761644
ATGCCGT967650.063.70142747
ATCTCGT956750.063.46024340
TATGCCG984700.063.38578846
TGCCGTC964000.063.3570848
GCCGTCT945900.063.34390649
CCGTCTT953950.063.30526450
CGTCTTC948450.062.78438651
GTATGCC1014250.061.83851245
CGTCTGA1127100.061.39705716
TTGAATC1049100.060.98995236
ACGTCTG1136500.060.8954415
ACACGTC1148200.060.43493313
GAATCTC1014500.060.3564238
CACGTCT1152200.060.16590514
GCTTGAA1009050.059.7310559
Produced by FastQC (version 0.11.2)