FastQCFastQC Report
Tue 8 May 2018
HWTTCBGX5_n01_mb96.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWTTCBGX5_n01_mb96.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21412854
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG2997631.399920813918593TruSeq Adapter, Index 3 (100% over 63bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG395600.062.48073243
TCTCGTA385450.062.47778741
CTCGTAT391100.062.3878542
CGTATGC398750.062.2566644
ATGCCGT398800.062.03592347
TATGCCG404450.061.4756746
TGCCGTC401600.061.42471348
GCCGTCT400650.061.33828749
CCGTCTT401950.061.2004250
ATCTCGT393550.061.13928640
CGTCTTC404650.060.56171451
CATCTCG398600.060.26907339
GTATGCC429950.057.94112445
GCTTGAA453950.054.14810659
CTTAGGC466550.053.97688733
TAGGCAT461000.053.53318435
TTAGGCA471200.053.1513834
ACTTAGG481350.052.68277432
CGTCTGA489400.052.5408916
TGCTTGA468100.052.377558
Produced by FastQC (version 0.11.2)